yo pro 1 pi apoptosis (Beyotime)
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Yo Pro 1 Pi Apoptosis, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yo+pro+1+pi+apoptosis/YO-PRO-1/pm41966552-153-14-20
Average 99 stars, based on 161 article reviews
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Flow Cytometry:Article Title: Nephropathogenic infectious bronchitis virus-induced pyroptosis of chicken renal tubular epithelial cells via the MDA5/NF-κB/NLRP3 signalling pathway Article Snippet: .. A Single Cell:Article Title: A biomimetic arsenic-based nanozyme enhances dual epigenetic regulation to improve the efficacy of immunotherapy for acute myeloid leukaemia. Article Snippet: Aberrant epigenetic modifications in acute myeloid leukaemia (AML) limit immunotherapeutic efficacy.. Arsenic ions are capable of undergoing valence transformation in organisms, exhibit favourable enzyme-mimetic activity, and thereby play a crucial role in enhancing dual epigenetic regulation.. Therefore, to achieve potent epigenetic regulation, in this study, a biomimetic arsenic-based nanozyme (As/ZIF-8@M) that exhibits leukaemia cell recognition and phagocytosis capabilities, as well as bone marrow homing effects, was engineered. Suspension:Article Title: A biomimetic arsenic-based nanozyme enhances dual epigenetic regulation to improve the efficacy of immunotherapy for acute myeloid leukaemia. Article Snippet: Aberrant epigenetic modifications in acute myeloid leukaemia (AML) limit immunotherapeutic efficacy.. Arsenic ions are capable of undergoing valence transformation in organisms, exhibit favourable enzyme-mimetic activity, and thereby play a crucial role in enhancing dual epigenetic regulation.. Therefore, to achieve potent epigenetic regulation, in this study, a biomimetic arsenic-based nanozyme (As/ZIF-8@M) that exhibits leukaemia cell recognition and phagocytosis capabilities, as well as bone marrow homing effects, was engineered. Staining:Article Title: A biomimetic arsenic-based nanozyme enhances dual epigenetic regulation to improve the efficacy of immunotherapy for acute myeloid leukaemia. Article Snippet: Aberrant epigenetic modifications in acute myeloid leukaemia (AML) limit immunotherapeutic efficacy.. Arsenic ions are capable of undergoing valence transformation in organisms, exhibit favourable enzyme-mimetic activity, and thereby play a crucial role in enhancing dual epigenetic regulation.. Therefore, to achieve potent epigenetic regulation, in this study, a biomimetic arsenic-based nanozyme (As/ZIF-8@M) that exhibits leukaemia cell recognition and phagocytosis capabilities, as well as bone marrow homing effects, was engineered. Article Title: The role of disulfidptosis-associated LncRNA-LINC01137 in Osteosarcoma Biology and its regulatory effects on macrophage polarization. Article Snippet: The objective of this research is to investigate the function of long non-coding RNA (lncRNA) associated with disulfidptosis, particularly LINC01137, in osteosarcoma (OS), and its impact on macrophage polarization and the tumor immune microenvironment (TME), with the goal of identifying new prognostic biomarkers and therapeutic targets.. Utilizing the OS transcriptome dataset from the TARGET database, differentially expressed lncRNAs related to disulfidptosis were identified.. The functional mechanisms of LINC01137, which affect cell proliferation, migration, invasiveness, programmed cell death, and macrophage orientation, were explored using the full suite of analyses provided by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), alongside a diverse array of laboratory experiments, including an in vivo osteosarcoma xenograft model in BALB/c nude mice to assess the impact of LINC01137 knockdown on tumor growth. Article Title: Paclitaxel and Cephalomannine Synergistically Induce PANoptosis in Triple-Negative Breast Cancer Through Oxygen-Regulated Cell Death Pathways Article Snippet: Secondary antibodies included Goat Anti-Mouse IgG (#7076S, CST, Danvers, MA, USA) and Goat Anti-Rabbit IgG (#7074P2, CST, Danvers, MA, USA). .. Other reagents included Pre-stained Protein Marker (26617, ThermoFisher Scientific, Waltham, MA, USA), WB ECL Chemiluminescence Reagent (32106, ThermoFisher Scientific, Waltham, MA, USA), SDS-PAGE Sample Buffer (ZS306, YEASEN, Shanghai, China), Quick PAGE Gel Preparation Kit (10%, pg112, YEASEN, Shanghai, China), Reactive Oxygen Species (ROS) Assay Kit (S0033S, Beyotime Biotechnology, Shanghai, China), Article Title: MiR-200a-3p/ZEB1/IRF1-mediated PANoptosis prompts Xiangshaliujunzi decoction to overcome 5-fluorouracil resistance in gastric cancer Article Snippet: The concentrations of IFN-γ, TNF-α, IL-1β, and IL-18 in the supernatant were determined using the Mouse IFN-γ ELISA Kit (D721025-0096, Sangon, China), Mouse TNF-α ELISA Kit (PT512, Beyotime, China), Mouse IL-1β ELISA Kit (PI301, Beyotime, China), and Mouse IL-18 ELISA Kit (PI553, Beyotime, China). .. Fluorescence staining for apoptosis, necroptosis, and pyroptosis detection Apoptosis, necroptosis, and pyroptosis were detected using the Incubation:Article Title: The role of disulfidptosis-associated LncRNA-LINC01137 in Osteosarcoma Biology and its regulatory effects on macrophage polarization. Article Snippet: The objective of this research is to investigate the function of long non-coding RNA (lncRNA) associated with disulfidptosis, particularly LINC01137, in osteosarcoma (OS), and its impact on macrophage polarization and the tumor immune microenvironment (TME), with the goal of identifying new prognostic biomarkers and therapeutic targets.. Utilizing the OS transcriptome dataset from the TARGET database, differentially expressed lncRNAs related to disulfidptosis were identified.. The functional mechanisms of LINC01137, which affect cell proliferation, migration, invasiveness, programmed cell death, and macrophage orientation, were explored using the full suite of analyses provided by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), alongside a diverse array of laboratory experiments, including an in vivo osteosarcoma xenograft model in BALB/c nude mice to assess the impact of LINC01137 knockdown on tumor growth. Binding Assay:Article Title: The role of disulfidptosis-associated LncRNA-LINC01137 in Osteosarcoma Biology and its regulatory effects on macrophage polarization. Article Snippet: The objective of this research is to investigate the function of long non-coding RNA (lncRNA) associated with disulfidptosis, particularly LINC01137, in osteosarcoma (OS), and its impact on macrophage polarization and the tumor immune microenvironment (TME), with the goal of identifying new prognostic biomarkers and therapeutic targets.. Utilizing the OS transcriptome dataset from the TARGET database, differentially expressed lncRNAs related to disulfidptosis were identified.. The functional mechanisms of LINC01137, which affect cell proliferation, migration, invasiveness, programmed cell death, and macrophage orientation, were explored using the full suite of analyses provided by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), alongside a diverse array of laboratory experiments, including an in vivo osteosarcoma xenograft model in BALB/c nude mice to assess the impact of LINC01137 knockdown on tumor growth. CCK-8 Assay:Article Title: Transcriptome analysis identifies the NR4A subfamily involved in the alleviating effect of folic acid on mastitis induced by high concentration of Staphylococcus aureus lipoteichoic acid Article Snippet: .. We used 10% fetal bovine serum (FBS, FS201-02, Transgen, Beijing, China), FA (IF0180, Solarbio, Beijing, China), aLTA (L2515, Sigma-Aldrich, St. Louis, Mo, USA), Cell Counting Kit-8 assay (CCK-8, CK04, Dojindo, Beijing, China), Article Title: Transcriptome analysis identifies the NR4A subfamily involved in the alleviating effect of folic acid on mastitis induced by high concentration of Staphylococcus aureus lipoteichoic acid. Article Snippet: .. We used 10% fetal bovine serum (FBS, FS201-02, Transgen, Beijing, China), FA (IF0180, Solarbio, Beijing, China), aLTA (L2515, Sigma-Aldrich, St. Louis, Mo, USA), Cell Counting Kit-8 assay (CCK-8, CK04, Dojindo, Beijing, China), Marker:Article Title: Paclitaxel and Cephalomannine Synergistically Induce PANoptosis in Triple-Negative Breast Cancer Through Oxygen-Regulated Cell Death Pathways Article Snippet: Secondary antibodies included Goat Anti-Mouse IgG (#7076S, CST, Danvers, MA, USA) and Goat Anti-Rabbit IgG (#7074P2, CST, Danvers, MA, USA). .. Other reagents included Pre-stained Protein Marker (26617, ThermoFisher Scientific, Waltham, MA, USA), WB ECL Chemiluminescence Reagent (32106, ThermoFisher Scientific, Waltham, MA, USA), SDS-PAGE Sample Buffer (ZS306, YEASEN, Shanghai, China), Quick PAGE Gel Preparation Kit (10%, pg112, YEASEN, Shanghai, China), Reactive Oxygen Species (ROS) Assay Kit (S0033S, Beyotime Biotechnology, Shanghai, China), Western Blot:Article Title: Paclitaxel and Cephalomannine Synergistically Induce PANoptosis in Triple-Negative Breast Cancer Through Oxygen-Regulated Cell Death Pathways Article Snippet: Secondary antibodies included Goat Anti-Mouse IgG (#7076S, CST, Danvers, MA, USA) and Goat Anti-Rabbit IgG (#7074P2, CST, Danvers, MA, USA). .. Other reagents included Pre-stained Protein Marker (26617, ThermoFisher Scientific, Waltham, MA, USA), WB ECL Chemiluminescence Reagent (32106, ThermoFisher Scientific, Waltham, MA, USA), SDS-PAGE Sample Buffer (ZS306, YEASEN, Shanghai, China), Quick PAGE Gel Preparation Kit (10%, pg112, YEASEN, Shanghai, China), Reactive Oxygen Species (ROS) Assay Kit (S0033S, Beyotime Biotechnology, Shanghai, China), SDS Page:Article Title: Paclitaxel and Cephalomannine Synergistically Induce PANoptosis in Triple-Negative Breast Cancer Through Oxygen-Regulated Cell Death Pathways Article Snippet: Secondary antibodies included Goat Anti-Mouse IgG (#7076S, CST, Danvers, MA, USA) and Goat Anti-Rabbit IgG (#7074P2, CST, Danvers, MA, USA). .. Other reagents included Pre-stained Protein Marker (26617, ThermoFisher Scientific, Waltham, MA, USA), WB ECL Chemiluminescence Reagent (32106, ThermoFisher Scientific, Waltham, MA, USA), SDS-PAGE Sample Buffer (ZS306, YEASEN, Shanghai, China), Quick PAGE Gel Preparation Kit (10%, pg112, YEASEN, Shanghai, China), Reactive Oxygen Species (ROS) Assay Kit (S0033S, Beyotime Biotechnology, Shanghai, China), Polyacrylamide Gel Electrophoresis:Article Title: Paclitaxel and Cephalomannine Synergistically Induce PANoptosis in Triple-Negative Breast Cancer Through Oxygen-Regulated Cell Death Pathways Article Snippet: Secondary antibodies included Goat Anti-Mouse IgG (#7076S, CST, Danvers, MA, USA) and Goat Anti-Rabbit IgG (#7074P2, CST, Danvers, MA, USA). .. Other reagents included Pre-stained Protein Marker (26617, ThermoFisher Scientific, Waltham, MA, USA), WB ECL Chemiluminescence Reagent (32106, ThermoFisher Scientific, Waltham, MA, USA), SDS-PAGE Sample Buffer (ZS306, YEASEN, Shanghai, China), Quick PAGE Gel Preparation Kit (10%, pg112, YEASEN, Shanghai, China), Reactive Oxygen Species (ROS) Assay Kit (S0033S, Beyotime Biotechnology, Shanghai, China), ROS Assay:Article Title: Paclitaxel and Cephalomannine Synergistically Induce PANoptosis in Triple-Negative Breast Cancer Through Oxygen-Regulated Cell Death Pathways Article Snippet: Secondary antibodies included Goat Anti-Mouse IgG (#7076S, CST, Danvers, MA, USA) and Goat Anti-Rabbit IgG (#7074P2, CST, Danvers, MA, USA). .. Other reagents included Pre-stained Protein Marker (26617, ThermoFisher Scientific, Waltham, MA, USA), WB ECL Chemiluminescence Reagent (32106, ThermoFisher Scientific, Waltham, MA, USA), SDS-PAGE Sample Buffer (ZS306, YEASEN, Shanghai, China), Quick PAGE Gel Preparation Kit (10%, pg112, YEASEN, Shanghai, China), Reactive Oxygen Species (ROS) Assay Kit (S0033S, Beyotime Biotechnology, Shanghai, China), Detection Assay:Article Title: Paclitaxel and Cephalomannine Synergistically Induce PANoptosis in Triple-Negative Breast Cancer Through Oxygen-Regulated Cell Death Pathways Article Snippet: Secondary antibodies included Goat Anti-Mouse IgG (#7076S, CST, Danvers, MA, USA) and Goat Anti-Rabbit IgG (#7074P2, CST, Danvers, MA, USA). .. Other reagents included Pre-stained Protein Marker (26617, ThermoFisher Scientific, Waltham, MA, USA), WB ECL Chemiluminescence Reagent (32106, ThermoFisher Scientific, Waltham, MA, USA), SDS-PAGE Sample Buffer (ZS306, YEASEN, Shanghai, China), Quick PAGE Gel Preparation Kit (10%, pg112, YEASEN, Shanghai, China), Reactive Oxygen Species (ROS) Assay Kit (S0033S, Beyotime Biotechnology, Shanghai, China), Bicinchoninic Acid Protein Assay:Article Title: Engineered cell membrane vesicles loaded with lysosomophilic drug for acute myeloid leukemia therapy via organ-cell-organelle cascade-targeting. Article Snippet: Acute myeloid leukemia (AML) presents significant treatment challenges due to the severe toxicities and limited efficacy of conventional therapies, highlighting the urgency for innovative approaches.. Organelle-targeting therapies offer a promising avenue to enhance therapeutic outcomes while minimizing adverse effects.. Herein, inspired that primary AML cells are enriched with lysosomes and sensitive to lysosomophilic drugs (e.g., LLOMe), we developed a smart nanodrug (Cas-CMV@LM) including the engineered cell membrane vesicles (CMVs) nanocarrier and the encapsulated drug cargo LLOMe (LM). Lysis:Article Title: Engineered cell membrane vesicles loaded with lysosomophilic drug for acute myeloid leukemia therapy via organ-cell-organelle cascade-targeting. Article Snippet: Acute myeloid leukemia (AML) presents significant treatment challenges due to the severe toxicities and limited efficacy of conventional therapies, highlighting the urgency for innovative approaches.. Organelle-targeting therapies offer a promising avenue to enhance therapeutic outcomes while minimizing adverse effects.. Herein, inspired that primary AML cells are enriched with lysosomes and sensitive to lysosomophilic drugs (e.g., LLOMe), we developed a smart nanodrug (Cas-CMV@LM) including the engineered cell membrane vesicles (CMVs) nanocarrier and the encapsulated drug cargo LLOMe (LM). Activity Assay:Article Title: Engineered cell membrane vesicles loaded with lysosomophilic drug for acute myeloid leukemia therapy via organ-cell-organelle cascade-targeting. Article Snippet: Acute myeloid leukemia (AML) presents significant treatment challenges due to the severe toxicities and limited efficacy of conventional therapies, highlighting the urgency for innovative approaches.. Organelle-targeting therapies offer a promising avenue to enhance therapeutic outcomes while minimizing adverse effects.. Herein, inspired that primary AML cells are enriched with lysosomes and sensitive to lysosomophilic drugs (e.g., LLOMe), we developed a smart nanodrug (Cas-CMV@LM) including the engineered cell membrane vesicles (CMVs) nanocarrier and the encapsulated drug cargo LLOMe (LM). Cytotoxicity Assay:Article Title: Engineered cell membrane vesicles loaded with lysosomophilic drug for acute myeloid leukemia therapy via organ-cell-organelle cascade-targeting. Article Snippet: Acute myeloid leukemia (AML) presents significant treatment challenges due to the severe toxicities and limited efficacy of conventional therapies, highlighting the urgency for innovative approaches.. Organelle-targeting therapies offer a promising avenue to enhance therapeutic outcomes while minimizing adverse effects.. Herein, inspired that primary AML cells are enriched with lysosomes and sensitive to lysosomophilic drugs (e.g., LLOMe), we developed a smart nanodrug (Cas-CMV@LM) including the engineered cell membrane vesicles (CMVs) nanocarrier and the encapsulated drug cargo LLOMe (LM). Fluorescence:Article Title: MiR-200a-3p/ZEB1/IRF1-mediated PANoptosis prompts Xiangshaliujunzi decoction to overcome 5-fluorouracil resistance in gastric cancer Article Snippet: The concentrations of IFN-γ, TNF-α, IL-1β, and IL-18 in the supernatant were determined using the Mouse IFN-γ ELISA Kit (D721025-0096, Sangon, China), Mouse TNF-α ELISA Kit (PT512, Beyotime, China), Mouse IL-1β ELISA Kit (PI301, Beyotime, China), and Mouse IL-18 ELISA Kit (PI553, Beyotime, China). .. Fluorescence staining for apoptosis, necroptosis, and pyroptosis detection Apoptosis, necroptosis, and pyroptosis were detected using the |
